Translational_Unit

Part:BBa_K622006:Experience

Designed by: Fumitaka Hashiya   Group: iGEM11_Kyoto   (2011-10-02)

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K622006

The function check of chitinase activity was performed by using DNS assay.

Since DNS assay is known as a method to detect the amount of reducing sugar, this assay was used to measure the quantity of N-acetylglucosamine, the main product of decomposition of chitin.

The chitinase activity was measured in the supernatant of culture. Figure 1 shows the amount of reducing sugar. From this result, no reducing sugar was observed and the chitinase activity couldn't observed.

Fig.1:

User Reviews

UNIQ0bb5b9e2bb849c04-partinfo-00000000-QINU UNIQ0bb5b9e2bb849c04-partinfo-00000001-QINU